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Goat Anti-HMGA1 (aa9-21) Antibody (EB09567)

Related Products: EBP06859 - Immunizing Peptide, EBP09567 - Immunizing Peptide, Goat Anti-HMGA1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09567 Goat Anti-HMGA1 (aa9-21) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09567 1
EB09567 (0.5µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
EB09567 2
EB09567 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09567 3
EB09567 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HMGA1, high mobility group AT-hook 1, HMG-R, HMGA1A, HMGIY, MGC12816, MGC4242, MGC4854, OTTHUMP00000016222, OTTHUMP00000016223, OTTHUMP00000016224, OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y
Official Symbol: HMGA1
Accession Number(s): NP_665906.1; NP_002122.1
Human GeneID(s): 3159
Non-Human GeneID(s): 15361 (mouse), 117062 (rat)
Important Comments: This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.

Immunogen

Peptide with sequence C-SQPLASKQEKDGT, from the internal region (near N Terminus) of the protein sequence according to NP_665906.1; NP_002122.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 24kDa band observed in Human Pancreas (calculated MW of 11.7kDa/10.7kDa according to NP_665906.1; NP_002122.1). This molecular weight is routinely observed by other sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skin and Placenta. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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