Change country
Home

Goat Anti-HSP60 / HSPD1 (aa333-344) Antibody (EB12834)

Code Name Applications Availability Size Tested species Price Grade  
EB12834 Goat Anti-HSP60 / HSPD1 (aa333-344) Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Rat, Pig $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB12834 1
EB12834 (0.03µg/ml) staining of Human Heart (A), Kidney (B) and Liver (C) lysate and in Pig Heart (D), Kidney (E) and Liver (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12834 2
EB12834 (0.1µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12834 3
EB12834 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB12834 4
EB12834 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB12834 5
EB12834 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HSPD1, heat shock 60kDa protein 1 (chaperonin), CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa chaperonin, 60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, chaperonin 60, heat shock protein 65, mitochondrial matrix prote
Official Symbol: HSPD1
Accession Number(s): NP_002147.2
Human GeneID(s): 3329
Important Comments: Reported variants represent identical protein: NP_955472.1, NP_002147.2.

Immunogen

Peptide with sequence C-NLEDVQPHDLGK, from the internal region of the protein sequence according to NP_002147.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 60kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1kDa according to Human NP_002147.2 and 60.9kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Dog, Pig, Cow

Product Reviews

Please login to review this product.