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Goat Anti-IDH3A Antibody (EB10995)

Related Products: EBP10995 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10995 Goat Anti-IDH3A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10995 1
EB10995 (0.1µg/ml) staining of Human Lymph Nodes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10995 2
EB10995 (0.1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10995 3
EB10995 (0.3µg/ml) staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10995 4
EB10995 (3.75µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: IDH3A, isocitrate dehydrogenase 3 (NAD+) alpha, H-IDH alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitric dehydrogenase subunit alpha
Official Symbol: IDH3A
Accession Number(s): NP_005521.1
Human GeneID(s): 3419
Non-Human GeneID(s): 67834 (mouse), 114096 (rat)

Immunogen

Peptide with sequence DFTEEICRRVKDLD, from the C Terminus of the protein sequence according to NP_005521.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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