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Goat Anti-IDH3B (aa33-46) Antibody (EB10998)

Related Products: EBP10997 - Immunizing Peptide, EBP10998 - Immunizing Peptide, Goat Anti-IDH3B (aa369-383) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10998 Goat Anti-IDH3B (aa33-46) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10998 1
EB10998 (0.3µg/ml) staining of Human Skeletal muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10998 2
EB10998 (2µg/ml) staining of Mouse Heart (A) and Mouse Muscle (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10998 3
EB10998 (0.1µg/ml) staining of Pig Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ11043, H-IDHB, MGC903, RP46, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit, isocitric dehydrogenase subunit beta
Official Symbol: IDH3B
Accession Number(s): NP_008830.2; NP_777280.1
Human GeneID(s): 3420
Non-Human GeneID(s): 170718 (mouse), 94173 (rat)
Important Comments: This antibody is expected to recognize isoform a (NP_008830.2) and isoform b (NP_777280.1).

Immunogen

Peptide with sequence C-HAASRSQAEDVRVE, from the internal region (near N Terminus) of the protein sequence according to NP_008830.2; NP_777280.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 39kDa band observed in Mouse, Rat and Pig Heart lysates and in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.9kDa according to NP_777280.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig,

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