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Goat Anti-IDH3B (aa369-383) Antibody (EB10997)

Related Products: EBP10997 - Immunizing Peptide, EBP10998 - Immunizing Peptide, Goat Anti-IDH3B (aa33-46) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10997 Goat Anti-IDH3B (aa369-383) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc
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EB10997 1
EB10997 (0.03µg/ml) staining of Mouse fetal Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ11043, H-IDHB, MGC903, RP46, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit, isocitric dehydrogenase subunit beta
Official Symbol: IDH3B
Accession Number(s): NP_008830.2; NP_777281.1
Human GeneID(s): 3420
Non-Human GeneID(s): 170718 (mouse), 94173 (rat)
Important Comments: This antibody is expected to recognize isoform a (NP_008830.2) and isoform c (NP_777281.1).

Immunogen

Peptide with sequence C-TTDFIKSVIGHLQTK, from the C Terminus of the protein sequence according to NP_008830.2; NP_777281.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40kDa band observed in lysates of Mouse fetal Kidney and fetal Skeletal Muscle and of cell line NIH3T3 (calculated MW of 42.2kDa according to Human NP_008830.2 and Mouse NP_570954.1). Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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