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Goat Anti-IDH3G (aa337-350) Antibody (EB11000)

Code Name Applications Availability Size Tested species Price Grade  
EB11000 Goat Anti-IDH3G (aa337-350) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat, Pig $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB11000 1
EB11000 (0.5µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11000 2
EB11000 (1µg/ml) staining of Rat (A) and Pig (B) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11000 3
EB11000 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: IDH3G, isocitrate dehydrogenase 3 (NAD+) gamma, H-IDHG, IDH-gamma, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD(+)-specific ICDH subunit gamma, NAD+-specific ICDH, OTTHUMP00000025984, OTTHUMP00000025987, OTTHUMP00000025988, isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit, isocitric dehydrogenase subunit gamma
Official Symbol: IDH3G
Accession Number(s): NP_004126.1; NP_777358.1
Human GeneID(s): 3421
Non-Human GeneID(s): 15929 (mouse), 25179 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004126.1; NP_777358.1).

Immunogen

Peptide with sequence C-DHLKLHSYATSIRK, from the internal region of the protein sequence according to NP_004126.1; NP_777358.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38kDa band observed in lysates of cell line MCF7 and of Rat and Pig Heart (calculated MW of 41.5kDa according to NP_777358.1). Recommended concentration: 0.5-2µg/ml.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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