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Goat Anti-ILK Antibody (EB05120)

Related Products: EBP05120 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05120 Goat Anti-ILK Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05120 1
EB05120 (0.5µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05120 2
EB05120 (1µg/ml) staining of HEK293 (A), A431 (B), HeLa (C) and Jurkat (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ILK, integrin-linked kinase, P59, DKFZp686F1765, ILK-2, 59 kDa serine/threonine-protein kinase, ILK-1, integrin-linked kinase-2, integrin-linked protein kinase, p59ILK
Official Symbol: ILK
Accession Number(s): NP_004508.1; NP_001014794.1; NP_001014795.1
Human GeneID(s): 3611
Important Comments: All 3 varients (represented by NP_004508.1; NP_001014794.1 and NP_001014795.1) encode the same protein.

Immunogen

Peptide with sequence C-DMIVPILEKMQDK, from the C Terminus of the protein sequence according to NP_004508.1; NP_001014794.1; NP_001014795.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 52kDa band observed in Mouse Kidney lysates and in lysates of cell line NIH3T3 and 50-51kDa in HEK293, A431, HeLa and Jurkat lysates (calculated MW of 51.4kDa according to Human NP_004508.1 and Mouse NP_034692.2). Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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