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Goat Anti-IRF5 Antibody (EB05585)

Code Name Applications Availability Size Tested species Price Grade  
EB05585 Goat Anti-IRF5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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EB05585 1
EB05585 staining (1µg/ml) Human Spleen (A) and A549 cell lysate (B) + peptide (C). (35µg protein in RIPA buffer). Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: IRF5, interferon regulatory factor 5, SLEB10
Official Symbol: IRF5
Accession Number(s): NP_116032.1; NP_001092097.2; NP_001092099.1
Human GeneID(s): 3663
Important Comments: This antibody is expected to recognize reported isoforms (NP_116032.1; NP_001092097.2 ; NP_001092099.1


Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_116032.1; NP_001092097.2; NP_001092099.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55+60kDa bands observed in Human Spleen lysates and approx 65kDa in lysates of cell line A549 (calculated MW of 57.8kDa according to NP_001092099.1 and 56.0kDa according to NP_116032.1; NP_001092097.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown). This data is from a previous batch, not on sale.

Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.This data is from a previous batch, not on sale.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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