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Goat Anti-IRF6 Antibody (EB05404)

Code Name Applications Availability Size Tested species Price Grade  
EB05404 Goat Anti-IRF6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05404 1
EB05404 staining (0.2µg/ml) of Human Ovary lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05404 2
HEK293 overexpressing IRF6 (RC201579) and probed with EB05404 (mock transfection in first lane), tested by Origene.
EB05404 3
EB05404 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05404 4
EB05404 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: IRF6, interferon regulatory factor 6, LPS, PIT, PPS, VWS, OFC6
Official Symbol: IRF6
Accession Number(s): NP_006138.1
Human GeneID(s): 3664
Non-Human GeneID(s): 54139 (mouse)

Immunogen

Peptide with sequence C-TPSMQLPPALPPQ, from the C Terminus of the protein sequence according to NP_006138.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6µg/ml.
IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration: 2-3µg/ml. Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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