Change country
Home

Goat Anti-Karyopherin (importin) beta 1 Antibody (EB07018)

Related Products: EBP07018 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07018 Goat Anti-Karyopherin (importin) beta 1 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human, Rat $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07018 1
EB07018 (0.03µg/ml) staining of A431 (A), HEK293 (B), Jurkat (C), (0.01ug/ml) Daudi (D), HeLa (E) and (0.1ug/ml) Kelly (F) and KNRK (G) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07018 2
EB07018 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear membrane, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07018 3
EB07018 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07018 4
EB07018 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: karyopherin (importin) beta 1, HGNC:6400, KPNB1, IMB1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97, importin 90, importin beta-1 subunit, karyopherin beta 1, nuclear factor p97, KapB1, IPO1
Official Symbol: KPNB1
Accession Number(s): NP_002256.2; NP_001263382.1
Human GeneID(s): 3837
Non-Human GeneID(s): 16211 (mouse), 24917 (rat)

Immunogen

Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2; NP_001263382.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90-95-kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Product Reviews

Please login to review this product.