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Goat Anti-LASS1 Antibody (EB08822)

Related Products: EBP08822 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08822 Goat Anti-LASS1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08822 1
EB08822 (1µg/ml) staining of Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: LAG1, CerS1, LASS1, LAG1 homolog, ceramide synthase 1, MGC90349, UOG1, LAG1 longevity assurance homolog 1, longevity assurance (LAG1, S. cerevisiae) homolog 1, longevity assurance gene 1, upstream of GDF1
Official Symbol: CERS1
Accession Number(s): NP_067090.1; NP_937850.1
Human GeneID(s): 10715
Non-Human GeneID(s): 93898 (mouse), 306351 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_067090.1and NP_937850.1).

Immunogen

Peptide with sequence C-KDLREYDTAEAQ, from the internal region of the protein sequence according to NP_067090.1; NP_937850.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 37 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 38.0 kDa according to NP_937850.1) An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Pig, Cow

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