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Goat Anti-MAN2A1 Antibody (EB08228)

Related Products: EBP08228 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08228 Goat Anti-MAN2A1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08228 1
EB08228 (0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08228 2
HEK293 overexpressing Man2A1 (RC220186) and probed with EB08228 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: MAN2A1, mannosidase, alpha, class 2A, member 1, GOLIM7, MANA2, MANII, Golgi alpha-mannosidase II, golgi integral membrane protein 7, mannosidase, alpha type II, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Official Symbol: MAN2A1
Accession Number(s): NP_002363.2
Human GeneID(s): 4124

Immunogen

Peptide with sequence C-KISSDIKSQNR, from the internal region of the protein sequence according to NP_002363.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml. An anonymous customer found positive results in WB on Human dermal fibroblasts.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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