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Goat Anti-MELK Antibody - Trial (EB05041-T)

Code Name Applications Availability Size Tested species Price Grade  
EB05041-T Goat Anti-MELK Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

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ARP American Research Products, Inc
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EB05041-T 1
EB05041 (0.1µg/ml) staining of Human Testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MELK, maternal embryonic leucine zipper kinase, KIAA0175, RP11-8N6.1, HPK38, OTTHUMP00000046113, pEg3 kinase
Official Symbol: MELK
Accession Number(s): NP_055606.1
Human GeneID(s): 9833


Peptide with sequence KRLVEDILSSCKV, from the C Terminus of the protein sequence according to NP_055606.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Experiments gave bands at approx 75kDa and 150kDa in Human Testis lysates and in cell lines HeLa and Jurkat after 0.1µg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3µg/ml. Preliminary testing was unsuccessful on Mouse Testis for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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