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Goat Anti-MEST Antibody (EB09351)

Related Products: EBP09351 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09351 Goat Anti-MEST Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09351 1
EB09351 (0.2µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MEST, mesoderm specific transcript homolog (mouse), DKFZp686L18234, MGC111102, MGC8703, PEG1, mesoderm specific transcript, paternally expressed gene 1
Official Symbol: MEST
Accession Number(s): NP_002393.2; NP_803490.1
Human GeneID(s): 4232
Non-Human GeneID(s): 17294 (mouse), 58827 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.

Immunogen

Peptide with sequence C-QELLYRYKQNRSGR, from the internal region of the protein sequence according to NP_002393.2; NP_803490.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 37kDa band observed in Mouse and Rat Testis lysates (calculated MW of 37.6kDa according to Human NP_803490.1 and 38.8kDa according to Rat NP_001009617.1). Recommended concentration: 0.2-0.5µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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