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Goat Anti-Munc13-4 / UNC13D (Internal) Antibody (EB07336)

Code Name Applications Availability Size Tested species Price Grade  
EB07336 Goat Anti-Munc13-4 / UNC13D (Internal) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc
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EB07336 1
EB07336 (2µg/ml) staining of Human T-lymphocyte lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07336 2
EB07336 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07336 3
EB07336 (0.5µg/ml) staining of paraffin embedded Mouse Lung (wt in A and KO in B) .
Click here to download PDF datasheet.

Target Protein

Principal Names: UNC13D, Munc13-4, unc-13 homolog D (C. elegans), FHL3, HLH3, HPLH3, unc-13 homolog D
Official Symbol: UNC13D
Accession Number(s): NP_954712.1
Human GeneID(s): 201294
Non-Human GeneID(s): 70450 (mouse), 192177 (rat)

Immunogen

Peptide with sequence C-ETQKHKKDLHPLFD, from the internal region of the protein sequence according to NP_954712.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5µg/ml. In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in Western blot on Human:
Dettmer V, Bloom K, Gross M, Weissert K, Aichele P, Ehl S, Cathomen T
Retroviral UNC13D Gene Transfer Restores Cytotoxic Activity of T Cells Derived from Familial Hemophagocytic Lymphohistiocytosis Type 3 Patients In Vitro.
Hum Gene Ther. 2019 Aug;30(8):975-984.
PMID: 31032638

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