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Goat Anti-MUNC18 / STXBP1 (isoform a) Antibody (EB06140)

Code Name Applications Availability Size Tested species Price Grade  
EB06140 Goat Anti-MUNC18 / STXBP1 (isoform a) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06140 1
EB06140 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06140 2
EB06140 (0.3µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06140 3
EB06140 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: STXBP1, MUNC18-1, UNC18, hUNC18, syntaxin binding protein 1, Hunc18, rbSec1, syntaxin-binding protein 1, RP11-56D16.3, EIEE4, FLJ37475, RBSEC1
Official Symbol: STXBP1
Accession Number(s): NP_003156.1
Human GeneID(s): 6812
Non-Human GeneID(s): 20910 (mouse)
Important Comments: This antibody is expected to recognize isoform a (NP_003156.1).

Immunogen

Peptide with sequence C-SRVSFEDQAPTME, from the C Terminus of the protein sequence according to NP_003156.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in Human Cerebellum and Mouse Brain lysates (calculated MW of 68.7kDa according to Human NP_003156.1 and Mouse NP_001107041.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse

Specific Reference

This antibody has been successfully used in IHC on Mouse:
Meijer M, Cijsouw T, Toonen RF, Verhage M.
Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons.
PLoS One. 2015 Sep 25;10(9):e0138950.
PMID: 26407320

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