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Goat Anti-NEK7 (aa 274-286) Antibody (EB10520)

Related Products: EBP10519 - Immunizing Peptide, EBP10520 - Immunizing Peptide, Goat Anti-NEK7 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10520 Goat Anti-NEK7 (aa 274-286) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10520 1
EB10520 (0.1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10520 2
EB10520 (0.3µg/ml) staining of Mouse Lung (A), Mouse Testis (B), Rat Lung (C) and Rat Testis (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NIMA (never in mitosis gene a)-related kinase 7, NIMA-related kinase 7, NEK7
Official Symbol: NEK7
Accession Number(s): NP_598001.1
Human GeneID(s): 140609
Non-Human GeneID(s): 59125 (mouse), 360850 (rat)

Immunogen

Peptide with sequence CINPDPEKRPDVT, from the C Terminus of the protein sequence according to NP_598001.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30-33kDa band observed in Human Lung and Testis lysates and in Mouse and Rat Lung and Testis lysates (calculated MW of 34.6kDa according to Human NP_598001.1 and 34.5kDa according to Mouse NP_067618.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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