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Goat Anti-NET1 / ARHGEF8 (Internal) Antibody (EB07268)

Related Products: EBP06148 - Immunizing Peptide, EBP07268 - Immunizing Peptide, Goat Anti-NET1 / ARHGEF8 (C Terminus) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07268 Goat Anti-NET1 / ARHGEF8 (Internal) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB07268 1
EB07268 (2.5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator
Official Symbol: NET1
Accession Number(s): NP_005854.2; NP_001040625.1
Human GeneID(s): 10276
Non-Human GeneID(s): 56349 (mouse), 307098 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Immunogen

Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2; NP_001040625.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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