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Goat Anti-Neurofascin (aa877-890) Antibody (EB10984)

Code Name Applications Availability Size Tested species Price Grade  
EB10984 Goat Anti-Neurofascin (aa877-890) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB10984 1
EB10984 (0.5µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NFASC, neurofascin, DKFZp686P2250, FLJ46866, KIAA0756, NF, NRCAML, OTTHUMP00000065316, OTTHUMP00000065319, OTTHUMP00000065323, OTTHUMP00000065324, neurofascin homolog
Official Symbol: NFASC
Accession Number(s): NP_001005388.2; NP_001153803.1; NP_001153804.1; NP_055905.2;
Human GeneID(s): 23114
Non-Human GeneID(s): 269116 (mouse), 116690 (rat)
Important Comments: This antibody is expected to recognize reported isoforms 1, 2, 3 and 4 (NP_001005388.2;,NP_001153803.1, NP_001153804.1and NP_055905.2 resp) .

Immunogen

Peptide with sequence C-NFSPNQTKFTVQRT, from the internal region of the protein sequence according to NP_001005388.2; NP_001153803.1; NP_001153804.1; NP_055905.2;.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 170kDa band observed in Human Lung lysates (calculated MW of 138kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem. 2009 Oct 16;284(42):28533-42. PMID: 19666467). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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