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Goat Anti-NUDC Antibody (EB09516)

Related Products: EBP09516 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09516 Goat Anti-NUDC Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09516 1
EB09516 (0.5µg/ml) staining of HeLa (A), HepG2 (B), K562 (C) and KNRK (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09516 2
EB09516 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: NUDC, nuclear distribution gene C homolog (A. nidulans), HNUDC, MNUDC, NPD011, OTTHUMP00000004405, nuclear distribution gene C homolog
Official Symbol: NUDC
Accession Number(s): NP_006591.1
Human GeneID(s): 10726
Non-Human GeneID(s): 18221 (mouse), 29648 (rat)

Immunogen

Peptide with sequence C-PNLGNGADLPNYR, from the internal region of the protein sequence according to NP_006591.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in lysates of cell lines HeLa. HepG2 and K562, and approx. 38kDa in lysates of cell line KNRK (calculated MW of 38.2kDa according to Human NP_006591.1) and 38.4kDa according to Rat NP_058967.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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