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Goat Anti-Nur77 / TR3 (isoform a) Antibody (EB07404)

Related Products: EBP07404 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07404 Goat Anti-Nur77 / TR3 (isoform a) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07404 1
EB07404 (2µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07404 2
EB07404 (5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NR4A1, NUR77, nuclear receptor subfamily 4, group A, member 1, GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, TR3, TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, orphan nuclear receptor HMR, steroid receptor TR3
Official Symbol: NR4A1
Accession Number(s): NP_002126.2; NP_775180.1
Human GeneID(s): 3164
Non-Human GeneID(s): 15370 (mouse), 79240 (rat)
Important Comments: This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.

Immunogen

Peptide with sequence C-GRLPSKPKQPPDAS, from the internal region of the protein sequence according to NP_002126.2; NP_775180.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Breast. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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