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Goat Anti-PALS2 / VAM1 / MPP6 Antibody (EB06226)

Related Products: EBP06226 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06226 Goat Anti-PALS2 / VAM1 / MPP6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06226 1
EB06226 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Detected by western blot using chemiluminescence.
EB06226 2
EB06226 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MPP6, VAM1, p55T, VAM-1, membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6), MAGUK protein p55T, VELI-associated MAGUK 1, protein associated with Lin7 2, PALS2
Official Symbol: MPP6
Accession Number(s): NP_057531.2
Human GeneID(s): 51678
Non-Human GeneID(s): 56524 (mouse)

Immunogen

Peptide with sequence QQVLENLTELPSS-C, from the N Terminus of the protein sequence according to NP_057531.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in lysates of cell line HEK293 (calculated MW of 61.1kDa according to NP_057531.2). Recommended concentration: 0.3-1µg/ml. Primary incubated was for 1 hour. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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