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Goat Anti-PCK1 / PEPCKC (internal) Antibody (EB06943)

Related Products: EBP06943 - Immunizing Peptide, EBP07755 - Immunizing Peptide, Goat Anti-PCK1 / PEPCKC (internal region) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06943 Goat Anti-PCK1 / PEPCKC (internal) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06943 1
EB06943 (2µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06943 2
EB06943 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06943 3
EB06943 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PCK1, phosphoenolpyruvate carboxykinase 1 (soluble), HGNC:8724, MGC22652, PEPCK1, PEPCKC, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, PEPCK-C
Official Symbol: PCK1
Accession Number(s): NP_002582.3
Human GeneID(s): 5105

Immunogen

Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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