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Code Name Applications Availability Size Tested species Price Grade  
EB06682-T Goat Anti-PCSK9 (C Terminus) Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06682-T 1
EB06682 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06682-T 2
EB06682 (5µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06682-T 3
EB06682 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin\/kexin type 9, LDLCQ1, NARC-1
Official Symbol: PCSK9
Accession Number(s): NP_777596.2
Human GeneID(s): 255738

Immunogen

Peptide with sequence CRSRHLAQASQELQ, from the C Terminus of the protein sequence according to NP_777596.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;14(9):1161-9, PMID: 15772090. A customer found positive results in WB on Human Liver.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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