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Code Name Applications Availability Size Tested species Price Grade  
EB05403 Goat Anti-PEBP1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05403 1
EB05403 (1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05403 2
EB05403 (5µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05403 3
EB05403 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PEBP1, phosphatidylethanolamine binding protein 1, HCNP, PBP, PEBP, RKIP, prostatic binding protein, RAF kinase inhibitor protein, Raf kinase inhibitory protein, hippocampal cholinergic neurostimulating peptide
Official Symbol: PEBP1
Accession Number(s): NP_002558.1
Human GeneID(s): 5037
Non-Human GeneID(s): 23980 (mouse)

Immunogen

Peptide with sequence C-DYVPKLYEQLSGK, from the C Terminus of the protein sequence according to NP_002558.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 20-22kDa band observed in A549, HepG2 and Human Liver lysate (predicted MW of 22kDa according to NP_002558.1). Recommended for use at 0.5-2µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody (previous batch) has been successfully used in Rat in the following paper:
Weitzdörfer R, Höger H, Shim KS, Cekici L, Pollak A, Lubec G.
Changes of hippocampal signaling protein levels during postnatal brain development in the rat.
Hippocampus. 2008;18(8):807-13.
PMID: 18493952

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