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Goat Anti-PICK1 Antibody - Trial (EB07467-T)

Related Products: EBP07467 - Immunizing Peptide, Goat Anti-PICK1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07467-T Goat Anti-PICK1 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07467-T 1
EB07467 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07467-T 2
EB07467 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07467-T 3
EB07467 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PICK1, protein interacting with PRKCA 1, MGC15204, PRKCABP, OTTHUMP00000028509, PRKCA-binding protein, protein interacting with C kinase 1, protein kinase C, alpha binding protein
Official Symbol: PICK1
Accession Number(s): NP_036539.1
Human GeneID(s): 9463
Non-Human GeneID(s): 18693 (mouse), 84591 (rat)
Important Comments: Reported variants represent identical protein (NP_036539.1; NP_001034672.1; NP_001034673.1).

Immunogen

Peptide with sequence C-HYNKLQADPKQ, from the internal region of the protein sequence according to NP_036539.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in Mouse Brain lysates (calculated MW of 46.6kDa according to NP_036539.1, NP_001034672.1and NP_001034673.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Colon and Prostate. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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