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Goat Anti-PLRG1 Antibody (EB05248)

Code Name Applications Availability Size Tested species Price Grade  
EB05248 Goat Anti-PLRG1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $324.00 Elite

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ARP American Research Products, Inc
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EB05248 1
EB05248 (0.1µg/ml) staining of HEK293 (A) and NIH3T3 (B) nuclear cell lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05248 2
EB05248 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PLRG1, pleiotropic regulator 1 (PRL1 homolog, Arabidopsis), PRL1, MGC110980, pleiotropic regulator 1 (PRL1, Arabidopsis homolog), pleiotropic regulator 1 (PRL1homolog, Arabidopsis), pleiotropic regulator 1 (PRL1, Arabidopsis homolog)
Official Symbol: PLRG1
Accession Number(s): NP_002660.1; NP_001188493.1
Human GeneID(s): 5356
Non-Human GeneID(s): 60376 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).

Immunogen

Peptide with sequence C-PVSWKPEIIKRKRF, from the C Terminus of the protein sequence according to NP_002660.1; NP_001188493.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse NP_058064.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody (previous batch) has been successfully used in WB and ICC on Human:
Llères D, Denegri M, Biggiogera M, Ajuh P, Lamond AI.
Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.
EMBO Rep. 2010 Jun;11(6):445-51.
PMID: 20467437

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