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Goat Anti-Prion Protein (143-153) Antibody - Trial (EB07297-T)

Related Products: EBP07297 - Immunizing Peptide, Goat Anti-Prion Protein (143-153) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07297-T Goat Anti-Prion Protein (143-153) Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07297-T 1
EB07297 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRNP, prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia), ASCR, CD230, CJD, GSS, MGC26679, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, CD230 antigen, major prion protein, prion protein, prion protein (p27-30) (Creutzfeld-Jakob disease Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia), prion protein PrP, prion-related protein
Official Symbol: PRNP
Accession Number(s): NP_000302.1
Human GeneID(s): 5621
Non-Human GeneID(s): 19122 (mouse), 24686 (rat)
Important Comments: Reported variants represent identical protein (NP_000302.1; NP_898902.1; NP_001073590.1; NP_001073591.1; NP_001073592.1).

Immunogen

Peptide with sequence C-SDYEDRYYREN, from the internal region of the protein sequence according to NP_000302.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 27.7kDa according to NP_000302.1 and NP_898902.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Cow

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