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Goat Anti-PRKCDBP Antibody (EB09572)

Related Products: EBP09572 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09572 Goat Anti-PRKCDBP Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09572 1
EB09572 (0.1µg/ml) staining of Human Adipose cell llysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09572 2
EB09572 (0.3µg/ml) staining of Mouse Ovary (A) and Rat Uterus (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09572 3
EB09572 (3.75µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRKCDBP, protein kinase C, delta binding protein, HSRBC, MGC20400, SRBC, sdr-related gene product that binds to c-kinase
Official Symbol: CAVIN3
Accession Number(s): NP_659477.2
Human GeneID(s): 112464
Non-Human GeneID(s): 109042 (mouse), 85332 (rat)

Immunogen

Peptide with sequence C-RRTGLQKVQSLR, from the internal region of the protein sequence according to NP_659477.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38kDa band observed in Human Adipose, in Mouse Ovary and Rat Uterus and in NIH3T3 lysates (calculated MW of 27.7kDa according to Human NP_659477.2, of 27.8kDa according to Mouse NP_082720.1 and of 27.9kDa according to Rat NP_604444.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ). Recommended concentration: 0.1-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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