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Goat Anti-PROKR2 Antibody (EB09682)

Related Products: EBP09682 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09682 Goat Anti-PROKR2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09682 1
EB09682 (0.1µg/ml) staining of Human Cerebellum (A), Rat Brain (B) and Rat Testis (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09682 2
EB09682 (1µg/ml) staining of Rat Stomach lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09682 3
EB09682 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PROKR2, prokineticin receptor 2, GPR73L1, GPR73b, GPRg2, KAL3, PKR2, dJ680N4.3, G protein-coupled receptor 73-like 1, OTTHUMP00000030192
Official Symbol: PROKR2
Accession Number(s): NP_658986.1
Human GeneID(s): 128674
Non-Human GeneID(s): 192649 (rat)

Immunogen

Peptide with sequence C-NGNTSFTPNFN, from the internal region of the protein sequence according to NP_658986.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45kDa band observed in Human Brain (Cerebellum), Testis and Thyroid Gland and in Rat Brain, Stomach and Testis lysates (calculated MW of 44.0kDa according to Human NP_658986.1 and 47.9kDa according to Rat NP_620434.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat

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