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Goat Anti-PU.1 Antibody (EB08429)

Related Products: EBP08429 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08429 Goat Anti-PU.1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08429 1
EB08429 (0.03µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08429 2
EB08429 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08429 3
EB08429 (3.75µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1, hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1
Official Symbol: SPI1
Accession Number(s): NP_001074016.1; NP_003111.2
Human GeneID(s): 6688
Non-Human GeneID(s): 366126 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).

Immunogen

Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1; NP_003111.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat

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