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Goat Anti-PXR / NR1I2 Antibody (EB09172)

Related Products: EBP09172 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09172 Goat Anti-PXR / NR1I2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09172 1
EB09172 (1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09172 2
EB09172 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PXR, NR1I2, nuclear receptor subfamily 1, group I, member 2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, SAR, SXR, orphan nuclear receptor, pregnane X receptor, steroid and xenobiotic receptor
Official Symbol: NR1I2
Accession Number(s): NP_003880.3; NP_071285.1; NP_148934.1
Human GeneID(s): 8856
Important Comments: This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).

Immunogen

Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3; NP_071285.1; NP_148934.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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