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Code Name Applications Availability Size Tested species Price Grade  
EB05315 Goat Anti-RACGAP1 / MgcRacGAP Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05315 1
EB05315 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB05315 2
EB05315 (1µg/ml) staining of A431 nuclear (A), Jurkat (B), Jurkat nuclear (C) and negative control Human Pancreas (D) lysate. (35µg protein in RIPA buffer) Detected by chemiluminescence.
EB05315 3
EB05315 (4µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB05315 4
EB05315 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4
Official Symbol: RACGAP1
Accession Number(s): NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1
Human GeneID(s): 29127
Important Comments: This antibody is expected to recognize all reported isoforms (NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_001119575.1, NP_001306932.1, NP_001306930.1, NP_001306933.1

Immunogen

Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.
IHC: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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