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Goat Anti-RBP1 Antibody (EB06671)

Related Products: EBP06671 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06671 Goat Anti-RBP1 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06671 1
EB06671 (2µg/ml) staining of NIH3T3 (A) and (0.5ug/ml) of U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06671 2
EB06671 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06671 3
EB06671 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding protein 1, cellular, retinol-binding protein 1, cellular, CRBPI
Official Symbol: RBP1
Accession Number(s): NP_002890.2
Human GeneID(s): 5947

Immunogen

Peptide with sequence VEGVVCKQVFKKVQ, from the C Terminus of the protein sequence according to NP_002890.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. This molecular weight is routinely observed by other sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1). Recommended concentration: 0.5-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

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