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Goat Anti-RPL23 Antibody (EB11851)

Related Products: EBP11851 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11851 Goat Anti-RPL23 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11851 1
EB11851 (0.1µg/ml) staining of Human Colorectal Cancer lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11851 2
EB11851 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11851 3
EB11851 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 60S ribosomal protein L17, 60S ribosomal protein L23, L23, MGC111167, MGC117346, MGC72008, OTTHUMP00000164151, OTTHUMP00000164153, ribosomal protein L17, ribosomal protein L23, rpL17, RPL23
Official Symbol: RPL23
Accession Number(s): NP_000969.1
Human GeneID(s): 9349
Non-Human GeneID(s): 65019 (mouse), 29282 (rat)

Immunogen

Peptide with sequence KECADLWPRIASN, from the C Terminus of the protein sequence according to NP_000969.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 16kDa band observed in lysates of Human Colorectal Cancer and of cell lines NIH3T3 and HeLa (calculated MW of 14.9kDa according to NP_000969.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil and Liver. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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