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Code Name Applications Availability Size Tested species Price Grade  
EB10829 Goat Anti-SETDB2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB10829 1
EB10829 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SETDB2, SET domain, bifurcated 2, C13orf4, CLLD8, CLLL8, DKFZp586I0123, DKFZp761J1217, KMT1F, OTTHUMP00000018418, chronic lymphocytic leukemia deletion region gene 8 protein, histone-lysine N-methyltransfera
Official Symbol: SETDB2
Accession Number(s): NP_114121.2; NP_001153780.1
Human GeneID(s): 83852
Non-Human GeneID(s): 239122 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).

Immunogen

Peptide with sequence C-QRRHAKTNSHSSALH, from the internal region of the protein sequence according to NP_114121.2; NP_001153780.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 81.9kDa according to NP_114121.2). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

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