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Goat Anti-SIAHBP1 / FIR Antibody (EB05683)

Related Products: EBP05683 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05683 Goat Anti-SIAHBP1 / FIR Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05683 1
EB05683 (0.1µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05683 2
EB05683 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05683 3
EB05683 (5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PUF60, SIAHBP1, FIR, fuse-binding protein-interacting repressor, RoBPI, siah binding protein 1, poly-U binding splicing factor PUF60, Ro ribonucleoprotein-binding protein 1, pyrimidine tract binding splicing factor, poly-U binding splicing factor 60KDa, FLJ31379, FBP interacting repressor, Ro ribonucleoprotein binding protein 1
Official Symbol: PUF60
Accession Number(s): NP_510965.1; NP_055096.2; NP_001129505.1
Human GeneID(s): 22827
Non-Human GeneID(s): 67959 (mouse)
Important Comments: This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1; NP_055096.2; NP_001129505.1)

Immunogen

Peptide with sequence C-YDQERFDNSDLSA, from the C Terminus of the protein sequence according to NP_510965.1; NP_055096.2; NP_001129505.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human lysates of HEK293 (calculated MW of 59.9kDa according to NP_510965.1 and 58.2 according to NP_055096.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Prostate and Skin. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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