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Goat Anti-Silver homologue / Pmel 17 (C-terminus) Antibody (EB07296)

Related Products: EBP07296 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07296 Goat Anti-Silver homologue / Pmel 17 (C-terminus) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB07296 1
EB07296 (7µg/ml) staining of paraffin embedded Human Melanoma. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07296 2
EB07296 Negative Control showing staining of paraffin embedded Human Melanoma, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: silver homolog (mouse), PMEL17, SILV, D12S53E, ME20, SI, SIL, gp100, Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog
Official Symbol: PMEL
Accession Number(s): NP_008859.1
Human GeneID(s): 6490
Non-Human GeneID(s): 20431 (mouse), 362818 (rat)

Immunogen

Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Melanoma. Recommended concentration: 6-7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

Specific Reference

This antibody (previous batch) has been successfully used in Western blot on Zebrafish:
Liu C, Yan W, Zhou B, Guo Y, Liu H, Yu H, Giesy JP, Wang J, Li G, Zhang X.
Characterization of a bystander effect induced by the endocrine-disrupting chemical 6-propyl-2-thiouracil in zebrafish embryos.
Aquat Toxicol. 2012 Aug 15;118-119:108-15. 
PMID: 22542736

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