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Goat Anti-SLC12A2 Antibody (EB09783)

Related Products: EBP09783 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09783 Goat Anti-SLC12A2 Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB09783 1
EB09783 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09783 2
EB09783 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB09783 3
EB09783 (3.75µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
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Target Protein

Principal Names: basolateral Na-K-Cl symporter, BSC, BSC2, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, MGC104233, NKCC1, solute carrier family 12 (sodium/potassium/chloride transporters), member 2, SLC12A2
Official Symbol: SLC12A2
Accession Number(s): NP_001037.1; NP_001243390.1
Human GeneID(s): 6558
Non-Human GeneID(s): 20496 (mouse), 83629 (rat)

Immunogen

Peptide with sequence C-NALQHSIRLSGVEDH, from the internal region of the protein sequence according to NP_001037.1; NP_001243390.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
IHC: In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow