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Goat Anti-SLC2A4 / GLUT4 Antibody (EB10148)

Code Name Applications Availability Size Tested species Price Grade  
EB10148 Goat Anti-SLC2A4 / GLUT4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613

EB10148 1
EB10148 (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10148 2
EB10148 (3.75µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: glucose transporter 4, GLUT4, insulin-responsive glucose transporter type 4, solute carrier family 2 (facilitated glucose transporter), member 4, SLC2A4
Official Symbol: SLC2A4
Accession Number(s): NP_001033.1
Human GeneID(s): 6517
Non-Human GeneID(s): 20528 (mouse), 25139 (rat)


Peptide with sequence C-TELEYLGPDEND, from the C Terminus of the protein sequence according to NP_001033.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997 Mar 14;272(11):7085-92.; PMID: 9054401). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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