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Goat Anti-SLC6A4 / 5HTT / SERT Antibody (EB06743)

Related Products: EBP06743 - Immunizing Peptide, EBP11315 - Immunizing Peptide, Goat Anti-SLC6A4 (aa605-618) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06743 Goat Anti-SLC6A4 / 5HTT / SERT Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06743 1
EB06743 (2µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06743 2
EB06743 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC6A4, SERT, solute carrier family 6 (neurotransmitter transporter, serotonin), member 4, HGNC:11050, 5-HTT, HTT, OCD1, hSERT, 5-hydroxytryptamine transporter, 5HT transporter, Na+/Cl- dependent serotonin transporter, serotonin transporter, sodium-dependent serotonin transporter, solute carrier family 6 member 4, 5-HTTLPR, OTTHUMP00000163633
Official Symbol: SLC6A4
Accession Number(s): NP_001036.1
Human GeneID(s): 6532

Immunogen

Peptide with sequence C-LHQGERETWGK, from the internal region of the protein sequence according to NP_001036.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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