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Goat Anti-SMARCA3 Antibody (EB05441)

Related Products: EBP05441 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05441 Goat Anti-SMARCA3 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05441 1
EB05441 staining (0.5µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
EB05441 2
EB05441 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: HLTF, SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SNF2-like 3, sucrose nonfermenting-like 3, helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor-1 regulator, SNF2L3
Official Symbol: HLTF
Accession Number(s): NP_003062.2; NP_620636.1; NP_001305863.1
Human GeneID(s): 6596
Non-Human GeneID(s): 20585 (mouse)
Important Comments: .

Immunogen

Peptide with sequence C-KQAKINEIRTLIDL, from the C Terminus of the protein sequence according to NP_003062.2; NP_620636.1; NP_001305863.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 125kDa band observed in nuclear lysates of cell line HeLa and in preliminary testing of A431 nuclear cell lysate (calculated MW of 114kDa according to NP_003062.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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