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Code Name Applications Availability Size Tested species Price Grade  
EB08409 Goat Anti-SPHK1 Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08409 1
EB08409 (2µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SPHK1, sphingosine kinase 1, SPHK, SK1, SKase
Official Symbol: SPHK1
Accession Number(s): NP_068807.2; NP_892010.2; NP_001136073.1
Human GeneID(s): 8877
Non-Human GeneID(s): 20698 (mouse), 170897 (rat)
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).

Immunogen

Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2; NP_892010.2; NP_001136073.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3µg/ml.

Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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