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Goat Anti-SRF Antibody (EB09543)

Related Products: EBP09543 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09543 Goat Anti-SRF Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09543 1
EB09543 (1µg/ml) staining of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA.
Click here to download PDF datasheet.

Target Protein

Principal Names: SRF, serum response factor (c-fos serum response element-binding transcription factor), MCM1, OTTHUMP00000039820
Official Symbol: SRF
Accession Number(s): NP_003122.1
Human GeneID(s): 6722
Non-Human GeneID(s): 20807 (mouse), 501099 (rat)

Immunogen

Peptide with sequence C-QASPSRDSSTDLTQ, from the internal region of the protein sequence according to NP_003122.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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