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Goat Anti-STAT3 (isoform 1 and 2) Antibody (EB05073)

Code Name Applications Availability Size Tested species Price Grade  
EB05073 Goat Anti-STAT3 (isoform 1 and 2) Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human, Mouse $324.00 Elite

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ARP American Research Products, Inc
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Email: info@arp1.com
www.arp1.com

EB05073 1
EB05073 (4µg/ml) staining of paraffin embedded Mouse Thymus. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05073 2
EB05073 Negative Control showing staining of paraffin embedded Mouse Thymus, with no primary antibody.
EB05073 3
EB05073 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and weak nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05073 4
EB05073 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05073 5
EB05073 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
Official Symbol: STAT3
Accession Number(s): NP_003141.2; NP_644805.1
Human GeneID(s): 6774
Non-Human GeneID(s): 20848 (mouse), 25125 (rat)
Important Comments: This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)

Immunogen

Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2; NP_644805.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary testing showed a band at approx 80-85kDa in A431 nuclear cell lysate after 2µg/ml antibody staining (calculated MW of 80kDa according to NP_003141.2; and NP_644805.1). Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Mouse Thymus shows preferential staining of medula over cortex. Recommended concentration: 4-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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FAQs

In which mouse tissues / cell lysates has the antibody been tested?

The antibody stained mouse thymic medulla tissue in IHC assays. In WB assays the antibody was tested in NIH3T3 lysates.

 

In which concentration did you use your antibody?

WB assays: 1µg/ml

IHC assays: 10µg/ml