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|EB05073||Goat Anti-STAT3 (isoform 1 and 2) Antibody||Pep-ELISA, IHC, IF, FC||100µg specific antibody in 200µl||Human, Mouse||$324.00|
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Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2; NP_644805.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
In which mouse tissues / cell lysates has the antibody been tested?
The antibody stained mouse thymic medulla tissue in IHC assays. In WB assays the antibody was tested in NIH3T3 lysates.
In which concentration did you use your antibody?
WB assays: 1µg/ml
IHC assays: 10µg/ml