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Goat Anti-Thioredoxin Reductase 1 Antibody (EB05510)

Related Products: EBP05510 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05510 Goat Anti-Thioredoxin Reductase 1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05510 1
EB05510 staining (0.1µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05510 2
EB05510 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05510 3
EB05510 (2.5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TXNRD1, thioredoxin reductase 1, TR1, TXNR, GRIM-12, MGC9145, TR, TRXR1, KM-102-derived reductase-like factor, oxidoreductase, thioredoxin reductase GRIM-12
Official Symbol: TXNRD1
Accession Number(s): NP_003321.2; NP_877393.1; NP_877419.1; NP_877420.1; NP_001087240.1
Human GeneID(s): 7296
Important Comments: This antibody is expected to recognise reported isoform 1 (NP_003321.2), isoform 2 (NP_877393.1; NP_877419.1; NP_877420.1) and isoform 3 (NP_001087240.1)

Immunogen

Peptide with sequence TKRSGASILQAGC, from the C Terminus of the protein sequence according to NP_003321.2; NP_877393.1; NP_877419.1; NP_877420.1; NP_001087240.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human Placenta lysates (predicted MW of 57kDa according to NP_003321). Recommended for use at 0.1-1µg/ml.
IHC: Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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