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Goat Anti-TIM3 / HAVCR2 Antibody (EB07113)

Related Products: EBP05339 - Immunizing Peptide, EBP07113 - Immunizing Peptide, Goat Anti-TIM3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07113 Goat Anti-TIM3 / HAVCR2 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07113 1
EB07113 (0.1µg/ml) staining of A549 (A) and (0.5ug/ml) HepG2 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07113 2
EB07113 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07113 3
EB07113 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3
Official Symbol: HAVCR2
Accession Number(s): NP_116171.3
Human GeneID(s): 84868

Immunogen

Peptide with sequence C-KWYSHSKEKIQN, from the internal region of the protein sequence according to NP_116171.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35kDa band observed in lysates of cell lines A549 and HepG2 and in preliminary testing of Jurkat and U937 cell lysate (calculated MW of 33.4kDa according to NP_116171.3). Recommended concentration:0.1-1µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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