Search
Type in product code or name:
Goat Anti-TIM50 Antibody (EB06513)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06513 | Goat Anti-TIM50 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
||||||||
Target Protein
Principal Names: TIMM50, TIM50L, translocase of inner mitochondrial membrane 50 homolog (yeast), Tim50-like protein, homolog of yeast Tim50, translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae), MGC102733, TIM50Official Symbol: TIMM50
Accession Number(s): NP_001001563.1
Human GeneID(s): 92609
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.
Immunogen
Peptide with sequence CDVRTVLEHYALEDD, from the internal region of the protein sequence according to NP_001001563.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 39kDa in Human Heart lysate (Calculated MW of 50.5kDa according to NP_001001563.1) . The observed molecular weight corresponds to earlier finding in literature with different antibodies (Guo et al, J Bio Chem 2004 Jun 4;279:24813-25; PMID: 15044455). Recommended concentration: 0.5-1.5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Specific Reference
This antibody (previous batch) has been successfully used in Western blot on Dog:
Marfè G, Tafani M, Fiorito F, Pagnini U, Iovane G, De Martino L.
Involvement of FOXO transcription factors, TRAIL-FasL/Fas, and sirtuin proteins family in canine coronavirus type II-induced apoptosis.
PLoS One. 2011;6(11).
PMID: 22087287