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Goat Anti-TRIM5 / RNF88 (alpha isoform) Antibody (EB07084)

Code Name Applications Availability Size Tested species Price Grade  
EB07084 Goat Anti-TRIM5 / RNF88 (alpha isoform) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07084 1
EB07084 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM5, tripartite motif-containing 5, HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Official Symbol: TRIM5
Accession Number(s): NP_149023.1
Human GeneID(s): 85363
Important Comments: This antibody is expected to recognise isoform alpha (NP_149023.1) only.

Immunogen

Peptide with sequence CNIEKNENYQPK, from the internal region of the protein sequence according to NP_149023.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_149023.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested:
Expected from sequence similarity: Human

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