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Code Name Applications Availability Size Tested species Price Grade  
EB06901 Goat Anti-TRPC6 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB06901 1
EB06901 (1µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06901 2
EB06901 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRPC6, transient receptor potential cation channel, subfamily C, member 6, HGNC:12338, TRP6, transient receptor potential channel 6, FLJ11098, FLJ14863, FSGS2, TRP6
Official Symbol: TRPC6
Accession Number(s): NP_004612.2
Human GeneID(s): 7225

Immunogen

Peptide with sequence C-EKLSMEPNQEETN, from the C Terminus of the protein sequence according to NP_004612.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 100kDa band observed in lysates of cell line A549 (calculated MW of 106kDa according to NP_004612.2). Preliminary experiments also showed 100kDa in Human Lung lysates and in lysates of cell line HEK293.This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Mouse PMID: 32882106.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in WB on Mouse:
Babar Murtaza, Aziz Hichami, Amira S Khan, Jiri Plesnik, Omar Sery, Alexander Dietrich, Lutz Birnbaumer, Naim A Khan
Implication of TRPC3 channel in gustatory perception of dietary lipids
Acta Physiol (Oxf). 2020 Sep 3;e13554.  doi: 10.1111/apha.13554.
PMID: 32882106

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